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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12001/4121

 
Title :[原著]経胃投与された塩化カドミウムのラット小腸における吸収について
Title alternative :Uptake of cadmium by the small intestinal absorptive cells of rats
Authors :田中, 重徳
川村, 公一
瑞慶覧, 朝盛
中河, 志朗
脇坂, 晟
皆川, 輿栄
田中, 礼子
中井, 健五
Authors alternative :Tanaka, Shigenori
Kawamura, Koichi
Zukeran, Chosei
Nakagawa, Shiro
Wakisaka, Akira
Minagawa, Koei
Tanaka, Reiko
Nakai, Kengo
Authors affiliation :琉球大学医学部解剖学第1講座
秋田大学医学部病理学第2講座
秋田大学医学部生化学第1講座
新潟大学教育学部
秋田大学医学部薬理学講座
Issue Date :1982
Publisher :琉球大学医学部
Type Local :雑誌掲載論文
Type (NII) :Journal Article
ISSN :0285-9270
Abstract :The electron histochemical method using oxine (8-hydroxyquinoline) as chelating agent was used to study the mechanism by which per gastrem administered cadmium (Cd) might be uptaken by the absorptive cells of the small intestine (SI) of rats. Six rats weighing 250-450 g, anticipatively 12 hours starved, received an intragastric injection of 250 mg/kg of aqueous cadmium chloride (experimental group), whereas 3 rats similarly starved were given merely an equivalent volume of pure water (controle group). The histochemical procedure which had so far provided the convincing results were as follows : the animals were per fused first with 80 ml of 0.14 M barbital sodium saturated with oxine (pH 7.2-7.4) transcardially; the same perfusate was simultaneously perfused transgastrically. This procedure was followed by the per fusion of 3 % glutaraldehyde in 0.14 M barbital sodium buffer saturated with oxine (pH 7.2-7.4). The sample were postfixed with 2% osmium tetroxide in 0.14 M barbital sodium saturated with oxine as mentioned, dehydrated in a graded series of ethyl alcohols or hexylene glycols, and finally embedded in Spurr's low viscosity resin. Electron micrograph revealed that in the lumen of the SI of experimental group, the reaction products appeared as linear profiles with high electron density. These were arranged more or less in parallel array, implying the existence of highly viscous substance which might contain Cd-oxine complexes. Typically, they were observed in close proximity to the microvilli of the SI absorptive cells; intramicrovillar spaces and pockets were filled with reticular profiles having a high electron density. Apical plasma membrane was occasionally observed to indent into the apical cytoplasm thus providing there profiles of tubular structure; numerous vesicles were found in the apical region of SI absorptive cells. These tubules and vesicles were found filled with the same substance of reticular structure as that of intramicrovillar spaces and pockets mentioned. The intracellular space was seen packed with massive electron dense material or with highly electron dense granules (EDG). There were found an abundance of EDG-containing vesicles dispersed throughout the cytoplasm of SI absorptive cells ; these EDG were also observed in the Golgi vesicles. The vesicles were also found in close association with the lateral and basal cell membranes. Interestingly, across these cell membrane the EDG were frequently found located opposite the EDG-containing vesicles inside the cells. These findings suggest that the EDG supposedly containing Cd-oxine complexes, might be excytosed in these sites of the cells. The mucosa of the SI was characterized by abundant migrating cells (leukocytes and macrophages), indicating that the transgastrical administration of Cd was accompanied by the cell infiltration in the mucosa of SI of rats. Noticeably, EDG were found distributed among these migrating cells and/or accumlated around the lymphatic as well as blood capillaries. Some migrating cells (macrophages) were seen containing in their cytoplasm a number of EDG aggregated. No profiles of EDG were observed in the specimens from the SI of rats of the controle group.
Rights :琉球医学会
URI :http://hdl.handle.net/20.500.12001/4121
Citation :琉球大学保健学医学雑誌=Ryukyu University Journal of Health Sciences and Medicine Vol.5 no.1 p.33 -47
Appears in Collections:Ryukyu University Journal of Health Sciences and Medicine

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