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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12001/23667

Title :Evaluation of candidate therapies using a patient-derived cervical cancer xenograft model
Authors :Nagayama-Urasoe, Chiaki
Umikawa, Masato
Asato, Tsuyoshi
Nagai, Yutaka
Aoki, Yoichi
Kariya, Ken-ichi
Authors affiliation :Department of Medical Biochemistry, Graduate School of Medicine
Issue Date :Dec-2017
Publisher :琉球医学会
Type Local :雑誌掲載論文
Type (NII) :Journal Article
ISSN :1346-888X
Abstract :Patient-derived xenograft (PDX) models are useful for preclinical evaluation of anticancer agents. However, establishing PDX models of cervical cancers are known to be challenging. We modified a protocol from the existing literature and established a model of an HPV16-positive squamous cell carcinoma on scid mice. The xenograft was positive for p16INK4a even after a passage, indicating the continued involvement of the E7 viral oncoprotein in abnormal cell growth. After 24 days of treatment with a nucleoside analog, gemcitabine, tumor growth was found to be suppressed in a dose-dependent manner, and the tumor became undetectable after high-dose treatment. Cediranib, an orally bioavailable inhibitor of neovascularization, reversed tumor growth until it was barely detectable. A hydrophobic cancer stem cell inhibitor, salinomycin, did not show any significant effect when used alone, but showed a tendency to act synergistically with low-dose gemcitabine. Although further procedural refinements are required, the model appeared to be useful for preclinical evaluation of various anticancer agents, including novel ones that target specific molecules.
Rights :琉球医学会
URI :http://hdl.handle.net/20.500.12001/23667
Citation :琉球医学会誌 = Ryukyu Medical Journal Vol.36 no.1-2 p.25 -28
Appears in Collections:Ryukyu Medical Journal

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